Unique MANUAL


- Author: Matteo Ramazzotti
- e-mail: matteo.ramazzotti@unifi.it
- download: Unique
- source code: code in pdf format.

Unique software have been written to find sequences that are repeated once or a precise number of times in DNA.

The Load Sequence Button
This is used to load a DNA sequence from a file. The DNA must be in plain text (no FASTA!) because a filter is applied in order to discard all non ATGCN characters.

The Paste Sequence Button
It is used to paste elsewhere copied DNA sequences. ATGCN filter is applied also to pasted sequence (see load button above).

The Scanning window Entry
The window is used to scan the sequence. e.g. Scanning Window = 6 will search for fragment of 6 bp stepping 1 base.

The Repetitions allowed Entry
It is the thereshold for output. Will be displayed only sequences of the selected lenght that are repeated a max of this threshold times. Setting the threshold to 1 only unrepeated sequences will be treated.

The Scan sequence Button
Run the scanning of the sequence.

Show Report/Hide Report
Will show/hide the report of the scanning. The results may be sorted by name or one can choose to view only unrepeated, all results or all but unrepeated sequences. Besides one can choose to view only palindromic sequences (if there are any, and only if the scanning window is even). If a sequence is selected in report window, with the locate button one can visualise it on the main sequence window.

Save Report
This will save the complete result on a selected .txt file

Good job

Matram